Characterisation of deubiquitylating enzymes in the cellular response to high-LET ionising radiation and complex DNA damage

Complex DNA damage (CDD) formation, which increases with increasing linear energy transfer (LET), is a major contributor to the therapeutic effect of radiotherapy. However little is known of the enzymes and mechanisms that control the cellular response to CDD and co-ordinate its repair. Using siRNA screening of deubiquitylating enzymes, we identify major roles for USP6 and ultimately PARP-1 protein in regulating CDD repair and in promoting cell survival in response to high-LET radiation.

Source: International Journal of Radiation Oncology * Biology * Physics - March 5, 2019 Category: Radiology Authors: Rachel J. Carter, Catherine M. Nickson, James M. Thompson, Andrzej Kacperek, Mark A. Hill, Jason L. Parsons Tags: Biology Contribution Source Type: research