PROTEOMIC ANALYSIS OF GLYCOSOMES FROM Trypanosoma cruzi EPIMASTIGOTES

In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite’s glycosomes.Graphical abstract
Source: Molecular and Biochemical Parasitology - Category: Parasitology Source Type: research