Suppression of lymphocyte apoptosis in spleen by CXCL13 after porcine circovirus type 2 infection and regulatory mechanism of CXCL13 expression in pigs

In this study, we found that the YL pigs showed more severe lymphocyte apoptosis and higher viral load in the spleen tissue than LW pigs. To illustrate the differential gene expression between healthy and infected spleens, transcriptome profiling of spleen tissues from PC V2-infected and control YL pigs was compared by RNA sequencing. A total of 90 differentially expressed genes (DEGs) was identified, includingCD207,RSAD2,OAS1,OAS2,MX2,ADRB3,CXCL13,CCR1, andADRA2C, which were significantly enriched in gene ontology (GO) terms related to the defense response to virus and cell –cell signaling, and another nine DEGs,KLF11,HGF,PTGES3,MAP3K11,XDH,CYCS,ACTC1,HSPH1, andRYR2, which were enriched in GO terms related to regulation of cell proliferation or apoptosis. Among these DEGs, theCXCL13 gene, which can suppress lymphocyte apoptosis during PCV2 infection, was significantly down-regulated in response to PCV2 infection in YL but not in LW pigs. By analysis of the regulatory elements in the promoter and 3 ′-untranslated region (3′-UTR) of porcineCXCL13, we found that the single nucleotide polymorphism (SNP) −1014 G (LW) >  A (YL) and theSus scrofa microRNA-296-5p (ssc-miR-296-5p) participated in regulatingCXCL13 expression during the response to PCV2 infection.
Source: Veterinary Research - Category: Veterinary Research Source Type: research