Genome and transcriptome analysis of Bacillus velezensisBS ‐37, an efficient surfactin producer from glycerol, in response to d‐/l‐leucine

The addition ofl‐Leu andd‐Leu to the culture medium had opposite effects on surfactin production by BS‐37. We sequenced the genome of BS‐37 and analyzed changes of the transcriptome in glycerol medium in response tod‐/l‐Leu. These results indicated thatl‐Leu has an important role as a precursor or substrate of peptide ring involved in surfactin production, whiled‐Leu acts as a competitive inhibitor AbstractSurfactin is one of the most widely studied biosurfactants due to its many potential applications in different fields. In the present study,Bacillus velezensis BS ‐37, initially identified as a strain ofBacillus subtilis, was used to efficiently produce surfactin with the addition of glycerol, an inexpensive by ‐product of biodiesel production. After 36 hr of growth in glycerol medium, the total surfactin concentration reached more than 1,000 mg/L, which was two times higher than that in sucrose medium. Moreover, the addition ofl‐ andd‐Leu to the culture medium had opposite effects on surfactin production by BS‐37. While surfactin production increased significantly to nearly 2,000 mg/L with the addition of 10 mMl‐Leu, it was dramatically reduced to about 250 mg/L with the addition of 10 mMd‐Leu. To systemically elucidate the mechanisms influencing the efficiency of this biosynthesis process, we sequenced the genome of BS‐37 and analyzed changes of the transcriptome in glycerol medium in response tod‐/l‐leucine. The RPKM analysis of ...

Source: MicrobiologyOpen - February 22, 2019 Category: Microbiology Authors: Dayuan Zhou, Fangxiang Hu, Junzhang Lin, Weidong Wang, Shuang Li Tags: ORIGINAL ARTICLE Source Type: research