Microinjection of 26RFa, an endogenous ligand for the glutamine RF-amide peptide receptor (QRFP receptor), into the Rostral Ventromedial Medulla (RVM), Locus Coelureus (LC), and Periaqueductal Grey (PAG) produces an analgesic effect in rats

Publication date: Available online 14 February 2019Source: PeptidesAuthor(s): Koji Yoshida, Takahiro Nonaka, Shingo Nakamura, Miki Araki, Tatsuo YamamotoAbstract26RFa is an endogenous ligand for the QRFP receptor. We previously found that intracerebroventricular injection of 26RFa produces an analgesic effect in a rat formalin test. In the present study, we directly tested the hypothesis that the analgesic effects of 26RFa in the formalin test are mediated in well-recognized regions of the descending inhibitory pain pathways, such as the rostral ventromedial medulla (RVM), locus coeruleus (LC), and periaqueductal grey (PAG) in rats. Injection cannulae were stereotaxically placed in the RVM, LC, or PAG through a burr hole. 26RFa (15 μg) or saline was delivered in a total volume of 0.5 μL. In a formalin test, 50 μL of 5% formalin was injected subcutaneously into the hind paw. In an antagonist study, idazoxan, an α-2 antagonist, or naloxone, an opioid receptor antagonist, was administered. Microinjection of 26RFa into the RVM had no effect compared with that in saline-injected rats. Microinjection of 26RFa into the LC contralateral, but not ipsilateral, to the formalin injection site significantly decreased the number of flinching behaviors compared with that of saline-injected rats. This effect was antagonized by intrathecal injection of idazoxan. Microinjection of 26RFa into the contralateral, but not ipsilateral, PAG produced an analgesic effect, and this effec...
Source: Peptides - Category: Biochemistry Source Type: research

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Abstract Every age group experiences back pain, a common reason for activity limitation. Management of back pain in a woman is challenging because of physiologic and hormonal changes through the woman's life cycle. The treatment is dictated by etiology and a woman's stage of life or reproductive events, such as pregnancy, breastfeeding, and menopause. Most episodes of low back pain are temporary with minimal long-term sequelae, but recurrent and long-lasting episodes require more thorough clinical evaluation. This monograph describes the anatomy of the spine; illustrates a focused, thorough evaluation, including o...
Source: Obstetrics and Gynecology - Category: OBGYN Authors: Tags: Obstet Gynecol Source Type: research
We present an unusual case of a large dumb-bell-shaped cold abscess arising due to infection of the sternum. A 23-year-old immunocompetent Asian woman presented with a gradually progressing painless swelling on anterior chest wall for the last 5  months. She had a large visible swelling on anterior chest wall which was 12.5 cm in diameter, soft, non-tender, temperature was not raised, and fluctuant. Magnetic resonance imaging showed a large dumb-bell-shaped hyperintense collection in upper anterior chest wall with marrow edema and corti cal irregularity in left side of manubrium. Pus was positive for nucleic ...
Source: Journal of Medical Case Reports - Category: General Medicine Source Type: research
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Source: Health News from Medical News Today - Category: Consumer Health News Tags: Sports Medicine / Fitness Source Type: news
Journal of Orthopaedic&Sports Physical Therapy, Ahead of Print.
Source: The Journal of Orthopaedic and Sports Physical Therapy - Category: Physiotherapy Authors: Source Type: research
Journal of Orthopaedic&Sports Physical Therapy, Ahead of Print.
Source: The Journal of Orthopaedic and Sports Physical Therapy - Category: Physiotherapy Authors: Source Type: research
Journal of Orthopaedic&Sports Physical Therapy, Ahead of Print.
Source: The Journal of Orthopaedic and Sports Physical Therapy - Category: Physiotherapy Authors: Source Type: research
Journal of Orthopaedic&Sports Physical Therapy, Ahead of Print.
Source: The Journal of Orthopaedic and Sports Physical Therapy - Category: Physiotherapy Authors: Source Type: research
Source: European Spine Journal - Category: Orthopaedics Source Type: research
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Source: European Spine Journal - Category: Orthopaedics Source Type: research
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