MicroRNA ‑124 improves functional recovery and suppresses Bax‑dependent apoptosis in rats following spinal cord injury.

MicroRNA‑124 improves functional recovery and suppresses Bax‑dependent apoptosis in rats following spinal cord injury. Mol Med Rep. 2019 Jan 28;: Authors: Xu Z, Zhang K, Wang Q, Zheng Y Abstract Spinal cord injury (SCI) induces aberrant expression of microRNAs (miRNAs), causing various secondary injury responses, including inflammation, apoptosis and oxidative stress. However, the mechanisms underlying miRNA‑mediated apoptosis have not been fully elucidated. In the present study, a rat SCI model was established and a miRNA microarray was analyzed to detect miRNA expression profiles at different times post‑SCI. The Basso, Beattie and Bresnahan score, cresyl violet staining and terminal deoxynucleotidyl‑-transferase‑mediated dUTP nick end labeling staining were used to evaluate locomotor activity, lesion volume and neuronal cell apoptosis, respectively, at different time points post‑SCI. It was observed that numerous miRNAs were altered at 14 days post‑SCI and miR‑124 was one of the most notably downregulated miRNAs. The present results demonstrated that overexpression of miR‑124 by agomir‑124 improves functional recovery, decreases lesion size and suppresses neuronal cell apoptosis in a rat SCI model. Luciferase reporter assay demonstrated that miR‑124 inhibited apoptosis regulator BAX (Bax) expression, a key molecule in the activation of the mitochondrial apoptotic pathway, by targeting its 3'‑untranslated ...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research