Nano polydopamine crosslinked thiol-functionalized hyaluronic acid hydrogel for angiogenic drug delivery

Publication date: Available online 22 January 2019Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Ramanathan Yegappan, Vignesh Selvaprithiviraj, Annapoorna Mohandas, Rangasamy JayakumarAbstractCrosslinking of polymeric network using nanoparticles via physical or chemical method to obtain hydrogel is an emerging approach. Herein, we synthesized polydopamine (PDA) nanoparticles via oxidative self-polymerization of dopamine in water-ethanol mixture. Thiol-functionalized hyaluronic acid was developed using cysteamine and hyaluronic acid (HA-Cys) via 1-Ethyl-3-(3-Dimethylaminopropyl) Carbodiimide - N-hydroxysuccinimide (EDC-NHS) crosslinking chemistry. Developed HA-Cys conjugate was cross-linked using PDA nanoparticles via Michael-type addition reaction. Synthesized nanoparticles were monodisperse with size of 124 ± 8 nm and had spherical morphology. FTIR characterization confirmed successful synthesis of HA-Cys conjugate and subsequent crosslinking with PDA nanoparticles. Rheological characterization revealed that hydrogels were injectable in nature with good mechanical stability. Dimethyloxalylglycine (DMOG) loaded PDA nanoparticle showed sustained drug release for period of 7 days from composite hydrogel. Endothelial cells, upon exposure to hydrogel’s microenvironment showed enhanced cell migration, proliferation and cell attachment. Further, in response to release of DMOG from developed hydrogel, cells showed enhanced capillary tube formation in vitro. Overal...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research