Photoswitching FRET to monitor protein-protein interactions [Cell Biology]

FRET is a powerful approach to study the interactions of fluorescent molecules, and numerous methods have been developed to measure FRET in cells. Here, we present a method based on a donor molecule’s photoswitching properties, which are slower in the presence vs. the absence of an acceptor. The technique, photoswitching...

Source: Proceedings of the National Academy of Sciences - January 15, 2019 Category: Science Authors: Kristin H. Rainey, George H. Patterson Tags: PNAS Plus Source Type: research