Development of a UPLC-ESI-MS method for simultaneous determination of flavonoids and diterpenes in Egletes viscosa (L.) Less herbal products

Publication date: Available online 8 January 2019Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Kaline Rodrigues Carvalho, Guilherme Julião Zocolo, Rita de Cássia Alves Pereira, Fátima Itana Chaves Custódio Martins, Paulo Riceli Vasconcelos Ribeiro, Edy Sousa de Brito, Edilberto Rocha Silveira, Kirley Marques CanutoAbstractEgletes viscosa (L.) Less (Asteraceae) is a tropical aromatic herb whose flower buds are used for gastrointestinal disorders. Its gastroprotective properties have been attributed to the flavonoid ternatin and the furan diterpenes tanabalin and centipedic acid. The aim of this study was to develop a method of ultra-performance liquid chromatography coupled to electrospray ionization and mass spectrometry (UPLC-ESI-MS) for identifying the constituents from E. viscosa flower buds as well as quantifying its bioactive compounds in herbal products. Infusions and tincture from wild and commercial E. viscosa materials were directly injected on a UPLC-quadrupole-time-of-flight system (UPLC-q-TOF). Afterwards, reference standards were used to quantify ternatin, tanabalin and centipedic acid in these samples, employing a UPLC-single quadrupole system set up for positive mode and Selected Ion Monitoring (SIM). Seventeen compounds were identified, including caffeic acid derivatives, flavonoids and diterpenes, from which seven have been reported for the first time in this specie. The quantification method showed good linearity (R > 0.99), acc...
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research