Novel Kdr mutations (K964R and A943V) in pyrethroid ‐resistant populations of Triatoma mazzottii and Triatoma longipennis from Mexico and detoxifying enzymes

AbstractAlthough having five different ways of transmission the vector ‐borne is the principal way of transmission of Chagas disease, which involves insects of the subfamily Triatominae (Hemiptera: Reduviidae). Nineteen of the 31 species that occur in Mexico are associated with humans, and all are capable of transmitting the disease. Pyrethroids are the insecticides recommended for the control of these vectors in Mexico. We determined the susceptibility to the pyrethroids deltamethrin and permethrin of peridomestic populations ofTriatoma mazzottii Usinger and two populations ofTriatoma longipennis Usinger in comparison with a reference strain for each species. Bioassays were performed for the determination of the LD50 for both field populations and reference strains. A maximum of 27 fold resistance to deltamethrin was observed inT. mazzottii, meanwhile, for permethrin,T. longipennis from Jalisco show the highest value of 3.19 fold. There was significantly increased activity of esterases in field populations in comparison with their corresponding reference strain. The results of the search ofkdr mutations related to the resistance to deltamethrin and permethrin in the evaluated species show the presence of mutations in the field populations, as is the case with individuals ofT. mazzottii, for which the mutation was found A943V, and for the two populations ofT. longipennis included in this study, we report the presence of thekdr mutation K964R. Evaluation of the various mecha...
Source: Insect Science - Category: Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research