An improved carbapenem inactivation method, CIMTrisII, for carbapenemase production by Gram-negative pathogens.

This study describes an improved carbapenem inactivation method, CIMTrisII, for detecting carbapenemase production by Gram-negative pathogens, including Enterobacteriaceae, Acinetobacter and Pseudomonas species. CIMTrisII was different from CIMTris in the concentration of Meropenem disks (5-µg MEM disks vs. 10-µg MEM disks), the inoculum volume of the bacteria (a 5-µl loopful vs. a 10 µl loopful) and the incubation time (1 vs. 2 h). CIMTrisII showed an overall sensitivity of 99.3 % and an overall specificity of 95.0 % for tested isolates. In comparison, CIMTris showed a sensitivity of 96.1 % and a specificity of 96.3 %, and mCIM showed a sensitivity of 67.1 % and a specificity of 100 %. CIMTrisII is thus deemed useful for detecting carbapenemase production by Gram-negative pathogens.
PMID: 30526741 [PubMed - as supplied by publisher]

Source: Journal of Medical Microbiology - December 10, 2018 Category: Microbiology Authors: Uechi K, Tada T, Kuwahara-Arai K, Sekiguchi JI, Yanagisawa I, Tome T, Nakasone I, Maeda S, Mya S, Zan KN, Tin HH, Kirikae T, Fujita J Tags: J Med Microbiol Source Type: research