Complex design of surgical instruments as barrier for cleaning effectiveness, favouring biofilm formation

ConclusionSoil and/or biofilms were evident on complex-design RSIs following 20 cycles of contamination and reprocessing, even using the reference standard method of cleaning. Although the depth gauges could be disassembled, biological residues and biofilm accumulated in its lumen. The current design of these RSIs prevents removal of all biological soil and this may have an adverse effect on patient outcome.
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research

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In this study, LysCSA13, an endolysin from S. aureus virulent bacteriophage CSA13, was cloned and characterized. LysCSA13 consists of an enzymatic active domain and a cell wall binding domain. LysCSA13 showed strong antimicrobial activity against staphylococcal strains at pH 7.0-9.0, 4.0-37.0 °C and in the presence of Ca2+ and Mn2+. In addition, a high efficacy of LysCSA13 in removing staphylococcal biofilms was observed on various surfaces, including polystyrene, glass and stainless steel, displaying an approximately 80-90% decrease in biofilm mass. Furthermore, 300 nM of LysCSA13 effectively removed staphylococca...
Source: Food Microbiology - Category: Food Science Authors: Tags: Food Microbiol Source Type: research
Publication date: Available online 19 August 2019Source: Microbial PathogenesisAuthor(s): Baifen Song, Jianxin Zhang, Jinzhu Ma, Zhenyue Feng, Liquan Yu, Yongzhong Yu, Yudong CuiAbstractThe purpose of this investigation was to construct a recombinant Escherichia coli strain displaying the Staphylococcus aureus target of RNAIII activating protein (TRAP) on its surface, and to investigate the strain for its immunogenicity. The lpp'ompA and lpp'ompA-TRAP genes were fused by the overlap polymerase chain reaction and then ligated into expression plasmid pQE30 producing pLO and pLO-TRAP. These two recombinant plasmids were trans...
Source: Microbial Pathogenesis - Category: Infectious Diseases Source Type: research
Publication date: Available online 16 August 2019Source: Journal of Ayurveda and Integrative MedicineAuthor(s): Sushma Bagde Bhatwalkar, Prashant Shukla, Rupesh K. Srivastava, Rajesh Mondal, Rajaneesh AnupamAbstractEnvironmental disinfection greatly reduces the occurrence of nosocomial or healthcare associated infections (HCAIs) which are the major healthcare problems worldwide. In India, Ayurvedic traditional fumigation with natural plant products is used to disinfect environment. In the present study, environmental disinfection efficiency of traditional fumigation practice has been evaluated by using natural plant produc...
Source: Journal of Ayurveda and Integrative Medicine - Category: Complementary Medicine Source Type: research
Publication date: Available online 16 August 2019Source: Polymer TestingAuthor(s): Natalia Sienkiewicz, Sylwia CzłonkaAbstractThis paper presents the effect of natural compound (E,E)-1,7-bis(4-Hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione (curcumin) on the antibacterial, anti-aging, mechanical and morphological properties of polyurethane (PU) rigid foams. Concentrations of 1 wt%, 2 wt% and 5 wt% of antibacterial compound was used, and the infection reduction capacity was evaluated for different types of bacteria (Escherichia coli (G-) and Staphylococcus aureus (G+). In order to determine all assumed properties t...
Source: Polymer Testing - Category: Chemistry Source Type: research
ConclusionThe microfractionation protocol developed in this study enabled time-efficient screening of many plants species, using a small quantity of sample material. In addition, microfractionation served as a guiding tool for identifying individual antimicrobial compounds. Through this process, flavonoids were isolated from Derris scandens, out of which dalpanithin and vicenin-3 showed activity in the low micromolar range. The high hit rate for in vitro antibacterial properties from this ethnopharmacologically guided sample collection gives credence to the Sri Lankan traditional herbal medicine as a source for drug discov...
Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
In this study, a gallium-loaded antimicrobial artificial dermal scaffold was successfully prepared by gallium ions and a collagen solution. The characterization results showed a porous structure with pore sizes ranging from 50 to 150 μm and a large porosity value of 97.4%. The enzymatic degradation rate in vitro was 19 and 28% after 12 and 24 h, respectively. In vitro antimicrobial testing revealed that the 1 h antibacterial rate against Staphylococcus aureus and Pseudomonas aeruginosa was close to 90%, which indicated its great antimicrobial activity. The results of the cytological evaluation showed slight effect...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
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Source: Journal of Saudi Chemical Society - Category: Chemistry Source Type: research
Publication date: 5 November 2019Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Volume 580Author(s): Elhamolsadat Ghorbi, Mehrnoush Namavar, Vahideh Rashedi, Saeideh Farhadinejad, Siamak Pilban Jahromi, Mohsen ZareianAbstractThe study investigated influence of copper oxide nanoparticle (CuO NP) concentrations on the bactericidal properties of silver-copper oxide (Ag/CuO) nanocomposite against Escherichia coli PTCC 1329, Klebsiella pneumoniae PTCC 1290 and Staphylococcus aureus PTCC 1189. Silver–copper oxide nanocomposite with varying concentrations of CuO was synthesized using a facile sol&...
Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - Category: Chemistry Source Type: research
In this study, positively charged hydroxyapatite – barium titanate composites showed antibacterial activity against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa with remarkable inhibition zone and moreover Confocal Laser Scanning Microscopy confirmed the reduction of cell counts in polarized samples from qualitative and antibacterial rate percentage reduction studies. These bactericidal and bacteria repelling action may be attributed to the positively polarized surface of the hydroxyapatite – barium titanate composites that rupture the bacteria membrane during culture.
Source: Materials Chemistry and Physics - Category: Materials Science Source Type: research
Conclusion: The antimicrobial properties of G. chinensis aqueous extract are likely related to its modulation of MRSA biofilm formation and carbohydrate metabolism. G. chinensis aqueous extract is a promising supplementary therapy to lessen or eliminate the use of antibiotics and is a potential tool for the management of MRSA infections. PMID: 31379958 [PubMed]
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
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