Antiproliferative and antiangiogenic effect of Amblyomma sculptum (Acari: Ixodidae) crude saliva in endothelial cells in vitro

Publication date: February 2019Source: Biomedicine & Pharmacotherapy, Volume 110Author(s): Paulo Luiz de Sá Junior, Diana Aparecida Dias Câmara, Juliana Mozer Sciani, Allan Saj Porcacchia, Pâmela Maria Moreira Fonseca, Ronaldo Zucatelli Mendonça, Selene Elifio-Esposito, Simone Michaela SimonsAbstractNeovascularization, a process that includes vasculogenesis and angiogenesis, may be a physiological or pathologic event, but in any cases the phenomenon is related to the formation of vascular net and sprouting of endothelial cells from preexisting blood vessel. The tumor environment, which counts on the tumor cell proliferation, is plenty of proangiogenic factors, such as angiogenin, TGF (α and β), FGF, VEGF, all of them playing a crucial role in angiogenesis, an important hallmark of cancer frequently related to a poor prognosis. Therefore, therapies focusing the inhibition of cancer neovasculogenesis have become an interesting strategy for the development of antitumor therapies. In this work, we investigate the effect of tick saliva on the human endothelial cells, in order to understand its inhibitory effects on angiogenesis. To this end, the HUVEC cells were used as model of angiogenesis in vitro and the anti-proliferative, anti-migratory, cytotoxicity was evaluated. Our data depicts that saliva impairs cell development by causing structural changes while precludes cell proliferation and migration, that are crucial events related to angiogenesis. Aiming the identificatio...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research