GSE123269 VEGAS: A Platform for Continuous Directed Evolution in Mammalian Cells (RNAseq)

Contributor : English G JustinSeries Type : Expression profiling by high throughput sequencingOrganism : Sindbis virusSindbis virus is a mutagenic ssRNA arbor virus. The structural genome component of this virus can be exchanged for any transgene of< 6kb in size. Here we sequence transgenic elements inserted to the virus. Our EGFP transgene was analyzed as a function of time during viral replication to quantify the mutagenic rate of the virus. Our nanobody transgene was analyzed to assess cDNA library complexity and identity.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Sindbis virus Source Type: research