Antibiofilm activity of three Actinomycete strains against Staphylococcus epidermidis

This study sought to identify novel and nontoxic biofilm inhibitors from theActinomycete library for attenuating biofilm formation byStaphylococcus epidermidis. After investigating the antibiofilm activities of spent media from 185Actinomycete strains using twoS.  epidermidis strains (ATCC 35984 and a clinical strain 5 ‐121‐2) as target bacteria, three strains of testedActinomycete (TRM 46200, TRM 41337, and TRM 46814) showed a significant inhibition againstS.  epidermidis biofilm formation without affecting the growth of planktonic cells. The characteristics of three strains of supernatants suggested that hydrophilic compound possibly extracellular peptides or proteins from these three strains, confer the biofilm reduction inS.  epidermidis. An attempt was made to assess their effects on biofilm components and cell surface hydrophobicities in order to disclose acting mechanisms. The crude proteins from spent media of three strains degraded not only exopolysaccharides but also extracellular DNA inS.  epidermidis biofilm. The active substances in crude proteins causedS.  epidermidis cells to become less hydrophobic. Given these results, the metabolites fromActinomycete strains should keep further attention as potential antibiofilm agents against biofilm formation ofS.  epidermidis, even biofilm infections of the other bacteria.Significance and Impact of the Study Staphylococcus epidermidis infections are frequently associated with biofilms that are difficult to eradi...
Source: Letters in Applied Microbiology - Category: Microbiology Authors: Tags: Review Article Source Type: research