Study of engineered low-modulus Mg/PLLA composites as potential orthopaedic implants: An in vitro and in vivo study

Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Xiaobing Yu, Wei Huang, Dewei Zhao, Ke Yang, Lili Tan, Xiuzhi Zhang, Junlei Li, Meng Zhang, Shuo Zhang, Tao Liu, Baolin Wu, Mingjia Qu, Ruimeng Duan, Yashuai YuanAbstractLow molecular weight poly-lactic acid (PLLA) is a polymer matrix of orthopaedic implants. The PLLA matrix incorporating bioactive magnesium ion (Mg2+) enhances bone regeneration. But the optimal ratio of Mg2+ to PLLA matrix has not been well reported and is worthy of study. We synthesized silane-coated Mg/PLLA composites containing 1%, 2%, 3%, 4% and 5% Mg micro-particles. The mechanical properties, in vitro cytocompatibility, cell viability and osteogenesis differentiation and in vivo performance of silane-coated Mg/PLLA composites were evaluated. These results showed that the bending and tensile strength of PLLA matrix was reduced by incorporation of Mg micro-particles. Mg/PLLA composites with higher Mg micro-particles ratio showed higher Mg2+ leaching rate and pH value in immersion solutions. MC3T3-E1 pre-osteoblasts incubated with Mg/PLLA composites containing higher ratio of Mg micro-particles showed higher cytocompatibility, cell viability, osteogenesis differentiation and migration. In vitro cellular responses showed that MC3T3-E1 pre-osteoblasts had the highest cell viability at 50 ppm Mg2+. In vivo animal studies showed there was no change in serum Mg2+ concentration after implanting Mg/PLLA compos...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research