SPIONs functionalized with small peptides for binding of lipopolysaccharide, a pathophysiologically relevant microbial product

Publication date: 1 February 2019Source: Colloids and Surfaces B: Biointerfaces, Volume 174Author(s): Weronika Karawacka, Christina Janko, Harald Unterweger, Marina Mühlberger, Stefan Lyer, Nicola Taccardi, Andriy Mokhir, Wolfgang Jira, Wolfgang Peukert, Aldo R. Boccaccini, Mikhail Kolot, Richard Strauss, Christian Bogdan, Christoph Alexiou, Rainer TietzeAbstractSystemic inflammation such as sepsis represents an acute life-threatening condition, to which often no timely remedy can be found. A promising strategy may be to functionalize magnetic nanoparticles with specific peptides, derived from the binding motives of agglutinating salivary proteins, that allow immobilization of pathogens. In this work, superparamagnetic iron oxide nanoparticles with stable polycondensed aminoalkylsilane layer were developed, to which the heterobifunctional linkers N-succinimidyl 3-(2-pyridyldithio)-propanoate (SDPD) and N-succinimidyl bromoacetate (SBA) were bound. These linkers were further chemoselectively reacted with the thiol group of singularly present cysteines of selected peptides. The resulting functional nanoparticles underwent a detailed physicochemical characterization. The biocompatibility of the primarily coated aminoalkylsilane particles was also investigated. To test the pathogen-binding efficacy of the particles, the lipopolysaccharide-immobilization capacity of the peptide-coated particles was compared with free peptides. Here, one particle-bound peptide species succeeded in...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research