MIR-144 Mediated NRF2 Gene Silencing Inhibits Fetal Hemoglobin Expression in Sickle Cell Disease

Inherited genetic modifiers and pharmacologic agents that enhance fetal hemoglobin (HbF) expression reverse the clinical severity of sickle cell disease (SCD). Recent efforts to develop novel strategies of HbF induction include discovery of molecular targets that regulate γ-globin gene transcription and translation. The purpose of this study was to perform genome-wide microRNA (miRNA) analysis identify genes associated with HbF expression in patients with SCD. We isolated RNA from purified reticulocytes for microarray-based miRNA expression profiling.

Source: Experimental Hematology - November 6, 2018 Category: Hematology Authors: Biaoru Li, Xingguo Zhu, Christina M. Ward, Athena Starlard-Davenport, Mayuko Takezaki, Amber Berry, Alexander Ward, Caroline Wilder, Cindy Neunert, Abdullah Kutlar, Betty S. Pace Source Type: research