Quantitative analysis of cell-free synthesized membrane proteins at the stabilized droplet interface bilayer.

We report the functional synthesis and quantification of membrane proteins-α-hemolysin from Staphylococcus aureus and the multidrug transporter EmrE from Escherichia coli-at the stabilized droplet interface bilayer using an in vitro transcription-translation system. The system developed here can expand the list of integral membrane proteins applicable for quantitative functional analysis.
PMID: 30310891 [PubMed - as supplied by publisher]

Source: Chemical Communications - October 12, 2018 Category: Chemistry Authors: Elfaramawy MA, Fujii S, Uyeda A, Osaki T, Takeuchi S, Kato Y, Watanabe H, Matsuura T Tags: Chem Commun (Camb) Source Type: research