Developmental Validation of the DNATyperTMY26 PCR Amplification Kit: An enhanced Y-STR multiplex for familial searching

Publication date: Available online 11 October 2018Source: Forensic Science International: GeneticsAuthor(s): Xiao-Ting Mo, Jian Zhang, Wen-Hua Ma, Xue Bai, Wan-Shui Li, Xing-Chun Zhao, Jian YeAbstractThe DNATyperTMY26 PCR Amplification kit, which including 26 low-medium mutating Y-STRs, is designed for Y-STR familial searching casework. The kit combines nine new Y-STR loci in addition to the 17 Y-STR loci from the commercially available AmpFlSTR®Yfiler® kit. The validation of the DNATyperTM Y26 kit was performed in terms of technical index, including accuracy, stability, species specificity, sensitivity, adaptability for various samples, and mixture. Further, mutations of the 26 Y-STRs were analyzed by 1167 DNA-confirmed father-son pairs, and the results indicated that these loci had low or medium mutation rates. Furthermore, these Y-STRs loci were also tested in 1072 random male samples from Henan, Shanxi, Inner Mongolia, and Chongqing in China, showing their high power for forensic discrimination in the Chinese population. Thus, the DNATyperTMY26 PCR Amplification kit is a powerful tool for ‘Y-STRs familial searching’ in actual sexual-assault cases, indicating its unique advantage in familial searching due to Y-STR loci with only low-medium mutation rates.

Source: Forensic Science International: Genetics - October 12, 2018 Category: Forensic Medicine Source Type: research