How high-resolution techniques enable reliable steroid identification and quantification

Publication date: Available online 27 September 2018Source: The Journal of Steroid Biochemistry and Molecular BiologyAuthor(s): Andrea Bileck, Christa E. Fluck, Nasser Dhayat, Michael GroesslAbstractDue to possible matrix interferences and artefact generation during sample preparation, careful method validation is required for quantitative bioanalytical methods, especially for analytes that are only present in low concentrations. Using the identification and quantification of progesterone metabolite in the urine of newborns as an example, we show how modern high-resolution instruments can be used to verify analyte assignment and avoid pitfalls commonly encountered by the use of low-resolution instruments.
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research