Fluorescence enhancement of silver nanocluster at intrastrand of a 12C-loop in presence of methylated region of sept 9 promoter.

Fluorescence enhancement of silver nanocluster at intrastrand of a 12C-loop in presence of methylated region of sept 9 promoter. Anal Chim Acta. 2018 Dec 14;1038:157-165 Authors: Sadeghan AA, Soltaninejad H, Hosseinkhani S, Hosseini M, Ganjali MR, Asadollahi MA Abstract Determining methylation state of a particular DNA sequence is an essential task in many epigenetic investigations. Here a facile method based on silver nanocluster (AgNCs) fluorescence enhancement is presented. Target sequences were selected from Sept9 promoter region that its hypermethylation is demonstrated as a reliable biomarker of colorectal cancer. Probe DNA was complementary to a 25 nucleotide of the target region and possessed twelve additional cytosines in the middle to grant the formation of AgNCs. After probe strands were hybridized with methylated and non-methylated targets separately, AgNCs were synthesized, and their fluorescence intensities were recorded. Fluorescence intensity enhanced when the target strands were methylated and quenched when they were non-methylated. The Linear range of fluorescence enhancement was from 1.0 × 10-7 M to 5.0 × 10-7 M with the detection limit of 7.6 × 10-8 M. Sensor specificity was checked with non-complementary strands with the maximum similarity of 40%. Further experiments explored various characteristics of methylated and non-methylated DNAs carrying AgNC and indicated that structure of methylated...
Source: Analytica Chimica Acta - Category: Chemistry Authors: Tags: Anal Chim Acta Source Type: research