GSE117373 Comparison of PPIL2 knockdown in HEK293T cells compared to scrambled shRNA control

Contributors : Tara L Davis ; S. R Jackson ; Beth Adams ; Anh Trinh ; Tracey Yenilaitus ; Jessica Kopenhaver ; Alyson Hurlock ; Angie Giang ; Hetty Rodriguez ; John TobiasSeries Type : Expression profiling by arrayOrganism : Homo sapiensHEK293T cells transduced with shRNA from MISSION library TRCN0000230475 using lentiviral delivery system. HEK293T cells transduced with scrambled shRNA, gifted from Dr. Mauricio Reginato. Tara L Davis, S. RaElle Jackson, Beth Adams, Anh Trinh, Tracey Yenailtus, Jessica Kopenhaver, Angie Giang, and Alyson Hurlock performed primary experimental contributions to cell lines, RNA/cDNA preparation, and validation, all Drexel University College of Medicine, Philadelphia, PA. Hetty Rodriguez and John Tobias performed Bioanalyzer and microarray expreriments, and initial data processing. Affiliation: Molecular Profiling Facility and Genomic Analysis Core Bioinformatics Group, University of Pennsylvania, Philadelphia, PA.Human PPIL2 is a cyclophilin, an enzyme that interconverts cis and trans isomers of proline. The PPIL2 gene, in addition to the cyclophilin domain, encodes for an N-terminal U-box motif. PPIL2 associates with the human spliceosome, the complex and dynamic machinery that removes intronic sequence from pre-messenger RNA (pre-mRNA). Nothing is known about the function of PPIL2 in regulation of alternative splicing, although it has been shown to modulate transcription of a handful of targets, albeit indirectly. To further understand ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research