The novel metallo- β -lactamase PNGM-1 from a deep-sea sediment metagenome: crystallization and X-ray crystallographic analysis

In this study, PNGM-1 was overexpressed, purified and crystallized. Crystals of native and selenomethionine-substituted PNGM-1 diffracted to 2.10 and 2.30   Å resolution, respectively. Both the native and the selenomethionine-labelled PNGM-1 crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 122, b = 83, c = 163   Å , β = 110 ° . Matthews coefficient (VM) calculations suggested the presence of 6 – 10 molecules in the asymmetric unit, corresponding to a solvent content of ∼ 31 – 58%. Structure determination is currently in progress.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: deep-sea sediment Edison Seamount metagenome antibiotic resistance metallo- β -lactamase research communications Source Type: research