Downregulation of peroxiredoxin II suppresses the proliferation and metastasis of gastric cancer cells.

Downregulation of peroxiredoxin II suppresses the proliferation and metastasis of gastric cancer cells. Oncol Lett. 2018 Oct;16(4):4551-4560 Authors: Niu L, Liu A, Xu W, Yang L, Zhu W, Gu Y Abstract Peroxiredoxin (Prx) II is an imperative member of the superfamily of peroxidases. It serves an essential role in scavenging organic hydroperoxide and H2O2. It is involved in the development of various malignant tumors. In order to investigate the significance of Prx II expressions level in gastric cancer (GC), downregulation of Prx II was performed to investigate its role in the proliferation and migration of gastric adenocarcinoma cells. In GC cells and 45 GC specimens, the mRNA and protein expression levels of Prx II were determined using reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis, respectively. The Prx II expression profile in another 116 GC specimens was also detected with immunohistochemistry (IHC). The changes in the proliferation and migration of MKN45 and MGC-803 cells folllowing transfection with small interfering RNA (siRNA) were detected by cell counting kit (CCK)-8, western blot analysis, and Transwell migration and invasion assays. The results revealed that the expression of Prx II in GC tissues and GC cells were significantly upregulated compared with the normal control. There was a significant association between the expression level of Prx II and various factors, includ...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research