Evaluation of free thyroxine determination based on one-step fluorometric immunoassay technique and the suboptimal concordance with two-step immunoassay

Publication date: Available online 26 July 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Radhouene Doggui, Rahma Mahjoub, Wassef Lotfi Aissa, Imen Tounsi, Zeineb Allagui, Zmorda Cherif, Sihem Haj Sassi, Sinda Ennigrou, Dhiaeddine Edriss, Jalila El Ati, Amina BibiAbstractFree thyroxine (FT4) quantification is continuing to be a concern. The purpose of the following study was to evaluate the analytical performance of Tosoh AIA900 based on a one-step technique and its comparison to Access 2 (two-step technique) over different clinical contexts (euthyroid, thyroid disorders, uncontrolled diabetes, renal failure and pregnancy). The protocol established by the French society of Clinical Biology was used to evaluate: imprecision, limit of detection, trueness, linearity, interferences and method comparisons. Within-run variation of 3.1%, 5.7% and 4.4% were found for the low, medium and high controls, respectively. Between-run was 5.8% for low control, 5.7% for medium control and 7.1% for high control. Common interferences did not affect one-step immunoassay FT4 results. The linearity was checked up to 86 pmol/L. The limit of detection was 5.5 pmol/L. The concordance correlation coefficient (CCC) showed a low agreement (0.6) between both methods. Bland-Altman plot revealed that AIA 900 one-step immunoassay technique provides a significant higher values ((+2.8 ± 2.7 pmol/L;p 
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Source: Monaldi Archives for Chest Disease - Category: Respiratory Medicine Tags: Monaldi Arch Chest Dis Source Type: research
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Source: Journal of the Academy of Nutrition and Dietetics - Category: Nutrition Source Type: research
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Source: Endocrinology and Metabolism Clinics of North America - Category: Endocrinology Authors: Source Type: research
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