Formation and nucleolytic processing of Cas9-induced DNA breaks in human cells quantified by droplet digital PCR.

In conclusion, the method we describe here can be used to study DSB repair mechanisms, with direct utility for molecularly optimising the knock-out/in outcomes in genome manipulation.
PMID: 30017059 [PubMed - in process]

Source: DNA Repair - July 19, 2018 Category: Genetics & Stem Cells Authors: Dibitetto D, La Monica M, Ferrari M, Marini F, Pellicioli A Tags: DNA Repair (Amst) Source Type: research