Gene expression patterns associated with human placental trophoblast differentiation

Publication date: Available online 10 January 2018Source: Clinica Chimica ActaAuthor(s): Shi-Wen Jiang, Wei Zhou, Jianhao Wang, Lauren M. Little, Lynn Leaphart, Jacob Jay, Eseosaserea Igbinigie, Haibin Chen, Jinping LiAbstractCell fusion is a hallmark of placental trophoblast cell differentiation and the mature syncytiotrophoblasts play essential roles for fetal-maternal exchange and production of pregnancy-related hormones. Using a well-established in vitro trophoblast differentiation model, we performed a microarray analysis on mRNA expression in trophoblast and syncytiotrophoblast cell cultures. Dramatic changes in gene expression patterns were detected during trophoblast differentiation. Real-time PCR analysis confirmed the reliability of the microarray data. As many as 3524 novel and known genes have been found to be up- or down-regulated for>2-fold. A number of cell cycle regulator including CDC6, CDC20, Cyclins B2, L1 and E2, were down-regulated in the syncytiotrophoblast, providing a mechanism for the loss of mitotic activity during trophoblast differentiation. Further characterization on the identified genes may lead to better understanding of placental patho-physiology in obstetric diseases such as preeclampsia.
Source: Clinica Chimica Acta - Category: Laboratory Medicine Source Type: research