Reduced inflammatory response by incorporating magnesium into porous TiO2 coating on titanium substrate

Publication date: Available online 17 July 2018Source: Colloids and Surfaces B: BiointerfacesAuthor(s): Xuezhong Li, Qianli Huang, Ling Liu, Weihong Zhu, Tarek A. Elkhooly, Yong Liu, Qingling Feng, Qingxiang Li, Shaoqiang Zhou, Yin Liu, Hong WuAbstractThe implant materials with proper anti-inflammatory and osteogenic properties may be promising for orthopedic applications. The inflammatory response induced by biomaterials has been regarded as one of the critical factors in determining in vivo fate of implants. Therefore, a novel bone biomaterial should have inflammation regulatory effects instead of being completely bio-inert. In the present work, the inflammation regulatory effects of exogenous magnesium (Mg) ions were investigated. Under the stimulation of lipopolysaccharide (LPS), macrophages exposed to Mg2+ exhibited down-regulated gene expressions of M1 markers (CD86, CD11c and inducible nitric oxide synthase (iNOS)) and pro-inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-1β), up-regulated gene expression of M2 marker CD163 and decreased TNF-α release, indicating that Mg2+ could switch macrophages from M1 to M2 phenotype. Thereafter, micro-arc oxidation (MAO) technique was employed to fabricate Mg-containing ceramic coatings on titanium substrates. Macrophages grown on Mg-containing surface were switched from M1 to M2 phenotype with the stimulation of LPS, evidenced by suppressed gene expressions of M1 markers (CD86, CD11c and iNO...
Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research