Inhibition of immunoproteasome promotes angiogenesis via enhancing hypoxia-inducible factor-1α abundance in rats following focal cerebral ischaemia

In this study, we identified that inhibition of immunoproteasome LMP2 was able to enhance angiogenesis and facilitate neurological functional recovery in rats after focal cerebral ischemia/reperfusion. In vitro, oxygen-glucose deprivation and reperfusion (OGD/R) significantly enhanced the expression of immunoproteasome LMP2 and proteasome activities in primary culture astrocytes, but these beneficial effects were abolished by knockdown of LMP2 with siRNA transfection. Along with this, protein abundance of HIF-1α was significantly increased by inhibition LMP2 in vivo and in vitro and was associated with angiogenesis and cell fates. However, these beneficial effects were partly abolished by HIF-1α inhibitor 2-methoxyestradiol (2ME). Taken together; this study highlights an important role for inhibition of LMP2 in promoting angiogenesis events in ischemic stroke, and point to HIF-1α as a key mediator of this response, suggesting that immunoproteasome inhibitors may be a promising strategy for stroke treatment.
Source: Brain, Behavior, and Immunity - Category: Neurology Source Type: research

Related Links:

In this study, we propose a deep convolution neural network for stroke magnetic resonance imaging(MRI) segmentation. The main structure of our network consists of two symmetrical deep sub-networks, in which dense blocks are embedded for extracting effective features from sparse pixels to alleviate the over-fitting problem of deep networks. We use the multi-kernel to divide the network into two sub-networks for acquiring more receptive fields, and the dropout regularization method to achieve an effective feature mapping. For the post-processing of the soft segmentation, we use image median filtering to alleviate noises and ...
Source: Neurocomputing - Category: Neuroscience Source Type: research
Authors: Anlıaçık SÖ, Tokgöz S, Zamani AG, Yıldırım MS, İyisoy MS Abstract Background/aim: We aimed to investigate the associations between endothelial nitric oxide synthase(eNOS) gene polymorphisms [G894T (rs1799983)], intron 4 (27-bpTR) variable number tandem repeat (VNTR) and T786C (rs2070744), and ischemic stroke in the Anatolian population. Materials and methods: This case-control study included 112 patients with “stroke of undetermined etiology” and 160 controls. Real-time polymerase chain reaction (RT-PCR) analysis was used to analyze these polymorphisms. Between-group ...
Source: Turkish Journal of Medical Sciences - Category: General Medicine Tags: Turk J Med Sci Source Type: research
CONCLUSION: In this retrospective single-center study undersized balloon angioplasty and deployment of a self-expandable stent with relatively low radial force was safe and effective for endovascular treatment of high-grade intracranial arterial stenosis with high technical success rate, low periprocedural complication rates and favorable medium-term follow-up results. PMID: 30991867 [PubMed - as supplied by publisher]
Source: Interventional Neuroradiology - Category: Radiology Tags: Interv Neuroradiol Source Type: research
Women who experience hypertensive disorders of pregnancy have an increased risk of developing cardiovascular diseases including hypertension, stroke, and ischaemic heart disease later in life.1 –3 This risk is evident shortly after an affected pregnancy and persists for decades.4 The mechanism underlying this association is not known, particularly whether women who develop hypertensive disorders are at higher risk pre-pregnancy and pregnancy unmasks their cardiovascular risk, or whether hypertensive disorders of pregnancy are an index event causing cardiovascular damage.
Source: European Journal of Obstetrics, Gynecology, and Reproductive Biology - Category: OBGYN Authors: Tags: Full length article Source Type: research
In the Reduction of Cardiovascular Events with Icosapent Ethyl –Intervention Trial (REDUCE-IT) [1], among patients with elevated triglycerides on statins, ischemic event risk was lower among those who received icosapent ethyl vs. placebo. However, while coronary events were reduced in both REDUCE-IT and another pure EPA trial, Japan EPA lipid intervention stu dy (JELIS) [2], ischemic stroke risk was lowered in REDUCE-IT (HR 0.64, 0.49-0.85), but not decreased in JELIS (HR 0.97, 0.85-1.10). The discrepancy between these two pure EPA trials might be due to the varied race-ethnicity composition of participants, since pa...
Source: Journal of the Neurological Sciences - Category: Neurology Authors: Tags: Letter to the Editor Source Type: research
It is undeniably the case that both diet and exercise influence the course of aging, though the size of the beneficial effect, even in the case of optimal lifestyle choices, is nowhere near as large as we'd all like it to be. Animal studies show calorie restriction extending maximum life span in mice by up to 40%, as well as lesser effects from various other forms of dietary strategy. Exercise meanwhile doesn't extend life span in mice, but does postpone age-related dysfunction and disease. Unfortunately, the effects on life span due to any of the strategies that are based on the metabolic effects of exercise and reduced c...
Source: Fight Aging! - Category: Research Authors: Tags: Medicine, Biotech, Research Source Type: blogs
Publication date: Available online 17 April 2019Source: Pharmacological ResearchAuthor(s): Ulvi BayraktutanABSTRACTStroke is classified into two main groups depending on its aetiology; ischaemic stroke and haemorrhagic stroke which successively develop from the occlusion or rupture of an artery leading to the brain. Despite being the leading cause of human cerebral damage, there is currently no medical therapy for haemorrhagic stroke and thrombolysis with recombinant tissue plasminogen activator remains the only approved pharmacotherapy for ischaemic stroke. However, due to its short therapeutic window (first 4.5 h ...
Source: Pharmacological Research - Category: Drugs & Pharmacology Source Type: research
ConclusionsIn patients with ACS, ticagrelor is more efficacious in protecting against new ischemic events and mortality than clopidogrel, irrespective of the presence of HF. There is no difference between ticagrelor or clopidogrel treatment in new-onset HF post-ACS.
Source: American Heart Journal - Category: Cardiology Source Type: research
Initial clinical results suggest that the new agent may be suitable for use with thrombolysis in acute ischemic stroke.Medscape Medical News
Source: Medscape Cardiology Headlines - Category: Cardiology Tags: Neurology & Neurosurgery News Source Type: news
Abstract For recanalization of emergent large vessel occlusions (ELVOs), endovascular therapy (EVT) using newer devices, such as a stent retriever and large-bore catheter, has shown better patient outcomes compared with intravenous recombinant tissue plasminogen activator only. Intracranial atherosclerotic stenosis (ICAS) is a major cause of acute ischemic stroke, the incidence of which is rising worldwide. Thus, it is not rare to encounter underlying ICAS during EVT procedures, particularly in Asian countries. ELVO due to underlying ICAS is often related to EVT procedure failure or complications, which can lead t...
Source: Korean J Radiol - Category: Radiology Authors: Tags: Korean J Radiol Source Type: research
More News: Brain | Ischemic Stroke | Neurology | Stroke | Study