Regulatory effects of microRNA ‑184 on osteosarcoma via the Wnt/β‑catenin signaling pathway.

Regulatory effects of microRNA‑184 on osteosarcoma via the Wnt/β‑catenin signaling pathway. Mol Med Rep. 2018 Jun 18;: Authors: Du Z, Li F, Wang L, Huang H, Xu S Abstract The present study aimed to investigate the role of microRNA (miRNA/miR)‑184 in osteosarcoma growth, development and metastasis, and the effects of miRNA‑184 on the proliferation, invasion and metastasis of osteosarcoma cells and associated mechanisms. In vitro, miR‑184 was transfected into U‑2OS cells and 143B cells. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to detect the expression of miR‑184. MTT was utilized to detect cell proliferation. A Transwell assay was applied to detect cell invasiveness. In vivo, an osteosarcoma tibial orthotopic metastatic tumor model was established, and western blotting and RT‑qPCR were used to detect the expression of Wnt and β‑catenin. Following the overexpression of miR‑184, the proliferation and cell invasion ability were significantly increased in U‑2OS and 143B cells. Following inhibition of miR‑184, cell proliferation and cell invasion ability were significantly decreased. In nude mice, tumor volume significantly increased following overexpression of miR‑184, and Wnt and phosphorylated β‑catenin levels were significantly increased. Following miR‑184 inhibition, tumor volume was significantly decreased, and Wnt and phosphorylated β‑catenin levels were...
Source: Molecular Medicine Reports - Category: Molecular Biology Tags: Mol Med Rep Source Type: research