Lability in sulfur acidic cultivation medium explains unstable effects of CDK inhibitors on Cyanidioschyzon merolae cell proliferation.

Lability in sulfur acidic cultivation medium explains unstable effects of CDK inhibitors on Cyanidioschyzon merolae cell proliferation. J Gen Appl Microbiol. 2018 Jun 14;: Authors: Kobayashi Y, Tanaka K Abstract We previously showed that nuclear DNA replication (NDR) is regulated by a checkpoint monitoring the occurrence of organelle DNA replication (ODR) in a unicellular red alga Cyanidioschyzon merolae. These analyses depended on the use of chemical CDK inhibitors such as CDK2 inhibitor II and roscovitine, but subsequent analyses yielded conflicting results depending on the experimental conditions. In the present study, we identified significantly short half-lives of the used chemicals in the sulfur acidic cultivation medium, which reconciles the discrepancy among these results. PMID: 29899184 [PubMed - as supplied by publisher]
Source: Journal of General and Applied Microbiology - Category: Microbiology Tags: J Gen Appl Microbiol Source Type: research