GSE114880 Next Generation Sequencing Facilitates Quantitative Analysis of H5N1 Wild Type and NS1-truncated Lung Transcriptomes in SPF chickens

Contributors : Shi Shaohua ; Peng DaxinSeries Type : Expression profiling by high throughput sequencingOrganism : Gallus gallusH5N1 highly pathogenic avian influenza (HPAI) has raised global concern for causing huge economic losses in poultry industry. By using reversal genetic technical, we have rescued a H5 wild-type virus rS and its NS1-truncated virus S-NS99, harboring only 99 amino acids in the amino terminus of NS1. We then used a high-throughput RNA-seq method to analyze host and pathogen transcriptomes in the lungs of chickens infected with rS and S-NS99. Sequenced numbers of viral transcripts and expression levels of host immune-related genes at 1 day post infection (dpi) were higher in rS-infected than S-NS99-infected chickens. The numbers of immune response of rS-infected chickens by Gene Ontology (GO) enrichment were higher than S-NS99-infected chickens. KEGG pathway analysis revealed rS infection could provoke significantly stronger immune response than S-NS99. Collectively, our data provide new insight into the underlying mechanisms of the di fferential length NS1 of avian influenza viruses.

Source: GEO: Gene Expression Omnibus - May 25, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Gallus gallus Source Type: research