Rapid and sensitive detection of Staphylococcus aureus assisted by polydopamine modified magnetic nanoparticles.

Rapid and sensitive detection of Staphylococcus aureus assisted by polydopamine modified magnetic nanoparticles. Talanta. 2018 Aug 15;186:147-153 Authors: Gao X, Yao X, Zhong Z, Jia L Abstract Pathogens cause significant morbidity and mortality to humans. Thus, development of fast and reliable methods for detection and identification of pathogens is urgently needed to increase protection level of public health and ensure the safety of consumers. Herein, a rapid and sensitive method has been developed for Staphylococcus aureus (S. aureus) detection based on the dual role of polydopamine modified magnetic nanoparticles (PDA@Fe3O4 NPs) combined with polymerase chain reaction (PCR) and capillary electrophoresis (CE). The core-shell type structure PDA@Fe3O4 NPs were prepared, which are spherical, about 152 ± 20 nm in diameter and the PDA shell is about 17.5 ± 1.6 nm. PDA@Fe3O4 NPs play a dual role including efficient capture of bacteria and extraction of DNA. In the pH range of 3.0-7.0, the capture efficiency of S. aureus by PDA@Fe3O4 NPs was more than 95% in 5 min. The adsorption capacity of the PDA@Fe3O4 NPs for S. aureus is 1.2 × 108 cfu mg-1. The efficient capture and concentration of bacteria from large volumes of samples by PDA@Fe3O4 NPs avoids the time-consuming culture-enrichment prior to PCR. Interestingly, PDA@Fe3O4 NPs were also found to be efficient adsorbents for extraction of genomic DNA from pathogens...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research