Angiostrongylus Cantonensis-Conditioned Culture Medium Induces Myelin Basic Protein Alterations via Erk1/2 and NF- κB Activation in Rat RSC 96 Schwann Cells.

Angiostrongylus Cantonensis-Conditioned Culture Medium Induces Myelin Basic Protein Alterations via Erk1/2 and NF-κB Activation in Rat RSC 96 Schwann Cells. Chin J Physiol. 2018 May 10;61 Authors: Chin JP, Chen CM, Lee TH, Chou CW, Lu CY Abstract Eating of excessive raw or undercooked environmental snails produces Angiostrongyliasis demyelination caused by Angiostrongylus cantonensis. The aim of this study was to investigate the association between extracellular signal-regulated kinase (Erk)1/2-nuclear factor (NF)-κB pathway and myelin basic protein (MBP) expression in RSC 96 Schwann cells treated with A. cantonensis- conditioned culture medium, which was prepared by culturing the third-stage (L3) nematode larvae in DMEM for 72 h. The supernatants were collected and filtered before use. Our results showed that MBP was produced in the RSC 96 cells at 16 h to 48 h post-stimulation (PS). Phosphorylated (p)- NF-κB levels were significantly increased from 8 h to 48 h PS, as were the p-Erk1/2 levels at the same time points. Additionally, expression of p-NF-κB and MBP was significantly decreased by treatment with QNZ, an NF-κB inhibitor. Treatment with PD98059, an Erk kinase inhibitor, efficiently reduced p-Erk1/2, p-NF-κB and MBP expression in the Schwann cells. These results suggest that A. cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-κB signaling pathway leading to reduced MBP production in RSC 96 Sc...
Source: The Chinese Journal of Physiology - Category: Physiology Tags: Chin J Physiol Source Type: research