Angiostrongylus Cantonensis-Conditioned Culture Medium Induces Myelin Basic Protein Alterations via Erk1/2 and NF- κB Activation in Rat RSC 96 Schwann Cells.
Angiostrongylus Cantonensis-Conditioned Culture Medium Induces Myelin Basic Protein Alterations via Erk1/2 and NF-κB Activation in Rat RSC 96 Schwann Cells.
Chin J Physiol. 2018 May 10;61
Authors: Chin JP, Chen CM, Lee TH, Chou CW, Lu CY
Abstract
Eating of excessive raw or undercooked environmental snails produces Angiostrongyliasis
demyelination caused by Angiostrongylus cantonensis. The aim of this study was to investigate the
association between extracellular signal-regulated kinase (Erk)1/2-nuclear factor (NF)-κB pathway
and myelin basic protein (MBP) expression in RSC 96 Schwann cells treated with A. cantonensis-
conditioned culture medium, which was prepared by culturing the third-stage (L3) nematode larvae
in DMEM for 72 h. The supernatants were collected and filtered before use. Our results showed that
MBP was produced in the RSC 96 cells at 16 h to 48 h post-stimulation (PS). Phosphorylated (p)-
NF-κB levels were significantly increased from 8 h to 48 h PS, as were the p-Erk1/2 levels at the same
time points. Additionally, expression of p-NF-κB and MBP was significantly decreased by treatment
with QNZ, an NF-κB inhibitor. Treatment with PD98059, an Erk kinase inhibitor, efficiently
reduced p-Erk1/2, p-NF-κB and MBP expression in the Schwann cells. These results suggest that A.
cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-κB signaling pathway
leading to reduced MBP production in RSC 96 Sc...
Source: The Chinese Journal of Physiology - Category: Physiology Tags: Chin J Physiol Source Type: research