New podolactones from the seeds of Podocarpus nagi and their anti-inflammatory effect

In this study, the seeds ofP. nagi were isolated by comprehensive chromatographic methods to obtain three new podolatones, named nagilactone B 1-O- β-d-glucoside (1), nagilactone N3 3-O- β-d-glucoside (2), and 2-epinagilactone B (3), as well as a known compound, nagilactone B (4). Their structures were determined by analyses of NMR and HRESIMS data. Compounds1 and2 significantly inhibited nitric oxide (NO) production on LPS-stimulated RAW264.7 macrophages, with IC50 values of 0.18  ± 0.04 and 0.53 ± 0.03 μM, respectively. Indomethacin (IC50 4.21  ± 0.32 μM) was used as a positive control. Compound1 suppressed the expression of inducible NO synthase (iNOS) in a concentration-dependent manner, mediating through inhibiting nuclear factor- κB (NF-κB) activity. This is the first report regarding the anti-inflammatory effect of podolactones, which could be potential anti-inflammatory agents.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research