Comparative analysis of immune responses by outer membrane antigens OMP10, OMP19 and OMP28 of   Brucella abortus.

Comparative analysis of immune responses by outer membrane antigens OMP10, OMP19 and OMP28 of  Brucella abortus. Jpn J Infect Dis. 2018 Apr 27;: Authors: Im YB, Park WB, Jung M, Kim S, Yoo HS Abstract Upon infection by Brucella, cytokine production is induced which becomes an important factor in evaluation of innate and adaptive immune responses. Several research groups have been investigating the mechanisms involved in Brucella infection within the host. Hence, we conducted an analytical study to define pathogenic pathways and immune mechanisms upon Brucella infection by investigating the efficacy of recombinant outer membrane protein 10 (rOMP10), outer membrane protein 19 (rOMP19) and outer membrane protein 28 (rOMP28) in in vitro and in vivo upon stimulation/immunization. Cytokine production was analyzed by NO assay and ELISA after stimulation of the proteins in RAW 264.7 cells and naïve splenocytes. Based on the data, the production levels of NO, TNF-α, and IL-6 increased time-dependently after having been stimulated with the proteins in the RAW 264.7 cells. On the other hand, the production of IFN-γ and IL-2 increased after stimulation of the proteins in naïve splenocytes. ELISA and ELISpot assays were used after the immunization with the proteins in mice. rOMP28 led to greater increases IFN-γ, IL-2, and TNF-α than IL-4 and IL-6 in vitro. Of the recombinant proteins, rOMP19 elicited mixed Th1/Th2 immune response by increa...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research