Bags versus flasks: a comparison of cell culture systems for the production of dendritic cell-based immunotherapies.

Bags versus flasks: a comparison of cell culture systems for the production of dendritic cell-based immunotherapies. Transfusion. 2018 Apr 19;: Authors: Fekete N, Béland AV, Campbell K, Clark SL, Hoesli CA Abstract In recent years, cell-based therapies targeting the immune system have emerged as promising strategies for cancer treatment. This review summarizes manufacturing challenges related to production of antigen presenting cells as a patient-tailored cancer therapy. Understanding cell-material interactions is essential because in vitro cell culture manipulations to obtain mature antigen-producing cells can significantly alter their in vivo performance. Traditional antigen-producing cell culture protocols often rely on cell adhesion to surface-treated hydrophilic polystyrene flasks. More recent commercial and investigational cancer immunotherapy products were manufactured using suspension cell culture in closed hydrophobic fluoropolymer bags. The shift to closed cell culture systems can decrease risks of contamination by individual operators, as well as facilitate scale-up and automation. Selecting closed cell culture bags over traditional open culture systems entails different handling procedures and processing controls, which can affect product quality. Changes in culture vessels also entail changes in vessel materials and geometry, which may alter the cell microenvironment and resulting cell fate decisions. Strategically desi...
Source: Transfusion - Category: Hematology Authors: Tags: Transfusion Source Type: research