Epiisopiloturine, an imidazole alkaloid, reverses inflammation and lipid peroxidation parameters in the Crohn disease model induced by trinitrobenzenosulfonic acid in Wistar rats

Publication date: June 2018 Source:Biomedicine & Pharmacotherapy, Volume 102 Author(s): Lucas Rodrigues de Carvalho, Tarcisio Vieira de Brito, José Simião da C. Júnior, Genilson José Dias Júnior, Diva de Aguiar Magalhãres, Stefany Guimarães Sousa, Renan Oliveira Silva, Felipe Rodolfo Pereira da Silva, Daniel Fernando Pereira Vasconcelos, Leiz Maria Costa Véras, José Roberto de Sousa de Almeida Leite, Dainesy Santos Martins, Conceição da Silva Martins, Jefferson Soares de Oliveira, André Luiz dos Reis Barbosa Epiisopiloturine (EPI) is an important imidazole alkaloid because of its pharmacological properties. The aim of this study was to investigate the effects of epiisopiloturine on inflammatory parameters of the colonic mucosa in a rat model of Crohn’s disease (CD). For this, we induced colitis using trinitrobenzenosulfonic acid and determined myeloperoxidase (MPO), interleukin 1 β (IL-1β), glutathione (GSH), and malondialdehyde (MDA) levels in the intestinal mucosa. The location and expression of the inflammatory markers in the colon were investigated by immunohistochemistry for NO synthase induced (iNOS), interleukin 1 beta (IL-1β), and cyclooxygenase-2 (COX-2) and western blotting (iNOS and COX-2), respectively. Compared with TNBS alone, epiisopiloturine at 1 mg/kg reduced the macroscopic and microscopic scores, wet weight of the colon, and neutrophilic infiltration and expression of the pro-inflammatory cytokine IL-1β. Epiisopi...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research