Transcriptome Analysis of ABA/JA-Dual Responsive Genes in Rice Shoot and Root.

Transcriptome Analysis of ABA/JA-Dual Responsive Genes in Rice Shoot and Root. Curr Genomics. 2018 Jan;19(1):4-11 Authors: Kim JA, Bhatnagar N, Kwon SJ, Min MK, Moon SJ, Yoon IS, Kwon TR, Kim ST, Kim BG Abstract The phytohormone abscisic acid (ABA) enables plants to adapt to adverse environmental conditions through the modulation of metabolic pathways and of growth and developmental programs. We used comparative microarray analysis to identify genes exhibiting ABA-dependent expression and other hormone-dependent expression among them in Oryza sativa shoot and root. We identified 854 genes as significantly up- or down-regulated in root or shoot under ABA treatment condition. Most of these genes had similar expression profiles in root and shoot under ABA treatment condition, whereas 86 genes displayed opposite expression responses in root and shoot. To examine the crosstalk between ABA and other hormones, we compared the expression profiles of the ABA-dependently regulated genes under several different hormone treatment conditions. Interestingly, around half of the ABA-dependently expressed genes were also regulated by jasmonic acid based on microarray data analysis. We searched the promoter regions of these genes for cis-elements that could be responsible for their responsiveness to both hormones, and found that ABRE and MYC2 elements, among others, were common to the promoters of genes that were regulated by both ABA and JA. These re...
Source: Current Genomics - Category: Genetics & Stem Cells Tags: Curr Genomics Source Type: research