Insertion of dengue E into lipid bilayers studied by neutron reflectivity and molecular dynamics simulations.

Insertion of dengue E into lipid bilayers studied by neutron reflectivity and molecular dynamics simulations. Biochim Biophys Acta. 2018 Feb 12;: Authors: Vanegas JM, Heinrich F, Rogers DM, Carson BD, La Bauve S, Vernon BC, Akgun B, Satija S, Zheng A, Kielian M, Rempe SB, Kent MS Abstract The envelope (E) protein of Dengue virus rearranges to a trimeric hairpin to mediate fusion of the viral and target membranes, which is essential for infectivity. Insertion of E into the target membrane serves to anchor E and possibly also to disrupt local order within the membrane. Both aspects are likely to be affected by the depth of insertion, orientation of the trimer with respect to the membrane normal, and the interactions that form between trimer and membrane. In the present work, we resolved the depth of insertion, the tilt angle, and the fundamental interactions for the soluble portion of Dengue E trimers (sE) associated with planar lipid bilayer membranes of various combinations of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-glycerol (POPG), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE), and cholesterol (CHOL) by neutron reflectivity (NR) and by molecular dynamics (MD) simulations. The results show that the tip of E containing the fusion loop (FL) is located at the interface of the headgroups and acyl chains of the outer leaflet of the lipid bilayers, in good agreement with prior predic...
Source: Biochimica et Biophysica Acta - Category: Biochemistry Authors: Tags: Biochim Biophys Acta Source Type: research

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Conclusion: The total subsample of laboratory exams is of great value, since it allowed us to establish national reference parameters adequate to sociodemographic and geographic characteristics of the Brazilian population, providing relevant and complementary information for the analysis of the health situation of Brazil.
Source: Revista Brasileira de Epidemiologia - Category: Epidemiology Source Type: research
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Source: Virus Research - Category: Virology Authors: Tags: Virus Res Source Type: research
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Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
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Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
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Source: Antiviral Therapy - Category: Virology Source Type: research
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Source: Antiviral Research - Category: Virology Authors: Tags: Antiviral Res Source Type: research
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Source: Biochimica et Biophysica Acta (BBA) Biomembranes - Category: Biochemistry Source Type: research
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Source: Reuters: Health - Category: Consumer Health News Tags: healthNews Source Type: news
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Source: EHP Research - Category: Environmental Health Authors: Tags: Research Articles February 2017 Source Type: research
In 2014, Behind the Headlines has covered more than 500 health stories that made it into the mainstream media. If you've been paying attention you should find this quiz easy and fun. Why not test your knowledge of 2014's health news with our month-by-month quiz? Answers are at the foot of the page (no peeking!).   In January 2016's health news... In a controversial study, monkeys were genetically engineered to develop what disorder? 1) Sex addiction 2) Bi-polar disorder 3) Autism In a similarly controversial study, what psychological condition was dismissed as a "myth" 1) Seasonal af...
Source: NHS News Feed - Category: Consumer Health News Tags: Special reports Source Type: news
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