A comparative analysis of RAPD and ISSR markers for assessing genetic diversity in Iranian populations of Nigella sativa L.

A comparative analysis of RAPD and ISSR markers for assessing genetic diversity in Iranian populations of Nigella sativa L. Cell Mol Biol (Noisy-le-grand). 2018 Jan 31;64(1):52-59 Authors: Hosseini KorehKhosravi S, Masoumiasl A, Dehdari M Abstract The genetic diversity of 18 Iranian populations of Nigella sativa L. was tested using RAPD (10 primers) and ISSR (12 primers) molecular markers. RAPD primers produced 103 acceptable bands and out of them, 42 bands were polymorphic and the total percentage of polymorphism was estimated 40.5%. Out of the 12 ISSR primers, only 5 of them produced acceptable bands. Five ISSR primers generated 39 amplified products and out of them, 19 bands were polymorphic and the total percentage of polymorphism was estimated 57.4%. As a result, the level of polymorphism obtained from the ISSR markers was higher than of the RAPD markers. Mean of marker index (MI) (0.822 for RAPD and 0.74 for ISSR) and Polymorphic information content (PIC) (0.402 for RAPD and 0.35 for ISSR) for each of the marker systems indicated that both markers were effective for evaluating genetic diversity. Cluster analysis classified the populations into 5 groups for both DNA markers. By integration and analyzing of the two marker systems, the populations were classified into 4 groups. The results of this research showed that RAPD and ISSR molecular markers can be useful for identifying and classifying the Iranian Nigella sativa L. POPULATIONS: PMID: ...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research

Related Links:

Peisheng Chen, Zilong Yao, Ganming Deng, Yilong Hou, Siwei Chen, Yanjun Hu, Bin Yu
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Emerging applications, such as gravitational wave astronomy, demand single-frequency lasers with diffraction-limited emission at 1.5   μ m. Fiber amplifiers have greatly evolved to fulfill these requirements. Hundreds of watts are feasible using large-mode-area and specialty fibers. ...
Source: Optics Letters - Category: Physics Authors: Source Type: research
A strong and dynamically controlled circular dichroism (CD) effect has aroused great attention due to its desirable applications in modern chemistry and life sciences. In this Letter, we propose a graphene – metal hybrid chiral metamaterial to generate mid-infrared CD with an intensity of more ...
Source: Optics Letters - Category: Physics Authors: Source Type: research
In this Letter, we experimentally demonstrate a 1550-nm direct modulation laser (DML)-based dual-band Nyquist PAM-4 intensity modulation direct detection (IMDD) system using a real-time field programmable gate array (FPGA)-based receiver. Although the sampling rate of the analog-to-digital ...
Source: Optics Letters - Category: Physics Authors: Source Type: research
The spatio-temporal evolution of the interaction of a high-power cylindrical vector (CV) beam with plasma is investigated numerically using the finite-difference time-domain (FDTD) method. According to the theory of ponderomotive nonlinearity of plasma, the Drude model with both modified plasma ...
Source: Journal of the Optical Society of America B - Category: Physics Authors: Source Type: research
Resonator Q-factor and amplifier flicker noise are the most important parameters that determine the phase noise of microwave oscillators. The Q-factor of intermediate frequency (IF) crystal resonators at IF frequencies can be larger than that of microwave resonators at microwave frequencies. In ...
Source: Journal of the Optical Society of America B - Category: Physics Authors: Source Type: research
Simulation of the most general configuration of a spaser where each gain element is assumed to have distinct behavior is almost intractable with current computing technologies. Hence, here we consider a representative, yet simple enough system with all possible interactions allowed, in which all ...
Source: Journal of the Optical Society of America B - Category: Physics Authors: Source Type: research
In this paper, the energy levels and the wave functions of the Schr ö dinger equation with position-dependent mass are theoretically deduced, and they are brought into nonlinear optical third-harmonic generation. We find that the peak of the third-harmonic coefficient becomes larger and a ...
Source: Journal of the Optical Society of America B - Category: Physics Authors: Source Type: research
PMID: 29784188 [PubMed - in process]
Source: Canadian Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Can J Ophthalmol Source Type: research
PMID: 29784187 [PubMed - in process]
Source: Canadian Journal of Ophthalmology - Category: Opthalmology Authors: Tags: Can J Ophthalmol Source Type: research
More News: Biology | Genetics | Iran Health | Molecular Biology