Akt activation improves microregional oxygen supply/consumption balance after cerebral ischemia-reperfusion.

Akt activation improves microregional oxygen supply/consumption balance after cerebral ischemia-reperfusion. Brain Res. 2018 Jan 22;: Authors: Weiss HR, Chi OZ, Kiss GK, Liu X, Damito S, Jacinto E Abstract There have been reports that activation of Akt may provide neuroprotection after cerebral ischemia-reperfusion. We tested the hypothesis that activation of Akt would decrease infarct size and improve microregional O2 supply/consumption balance after cerebral ischemia-reperfusion. This hypothesis was tested in isoflurane-anesthetized rats with middle cerebral artery blockade for 1 hr and reperfusion for 2 hr with or without SC-79 (Akt activator, 0.05 mg/kg, three doses). Regional cerebral blood flow was determined using a C14-iodoantipyrine autoradiographic technique. Regional small vessel (20-60 μm diameter) arterial and venous oxygen saturations were determined microspectrophotometrically. Akt phosphorylation was determined by Western blot. There were no significant hemodynamic or blood gas differences between groups. The control ischemic-reperfused cortex had a similar O2 consumption, but lower blood flow and higher O2 extraction compared to the contralateral cortex. However, microregional O2 supply/consumption balance was significantly reduced in the ischemic-reperfused cortex with many areas of low O2 saturation (42 of 80 veins with O2 saturation below 50%). SC-79 did not significantly affect cerebral O2 consumption, but signi...
Source: Brain Research - Category: Neurology Authors: Tags: Brain Res Source Type: research