Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing.
Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing.
Methods Mol Biol. 2018;1708:123-136
Authors: Miura F, Ito T
Abstract
Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.
PMID: 29224142 [PubMed - in process]
Source: Molecular Medicine - Category: Molecular Biology Authors: Miura F, Ito T Tags: Methods Mol Biol Source Type: research