Cytotoxic activities of some Turkish medicinal plants against HeLa cells < em > in vitro < /em >

Title: Cytotoxic activities of some Turkish medicinal plants against HeLa cellsin vitro Authors: Bozkurt-Guzel, Cagla; Serbetci, Tuba; Kultur, Sukran Abstract: The aim of this study was to characterize the biological activities of traditionally used medicinal plants generally collected from K ırklareli, Turkey against cancer. We evaluated the cytotoxic activities of different extracts prepared fromUrtica dioica L.1,Achillea millefolium L. ssp.pannonica2,Malva sylvestris L.3,Stachys cretica L. ssp .mersinaea (Boiss.) Rechf.f. (endemic)4,Melissa officinalis L.5,Cotinus coggyria Scop.6,Sorbus aucuparia L.7 , andPlantago major L. ssp.major8 species. Ethanol(a), petroleum ether(b), dichloromethane(c) and ethyl acetate(d) fractions of each plant material were obtained. Cytotoxicity in HeLa cells was evaluated using the MTT assay. Among the extracts, 7c, 2b, 6d, 7d, 7a, 2c, and 3b showed potent cytotoxic activity with IC50 values of less than 50 μg/mL, in descending order. Dichloromethane extract ofS. aucuparia is considered highly active, with IC50 of 15 ±03 (mean ± SD) μg/mL. The extracts 8a and 8b also showed good cytotoxic activity. According to the results, dichloromethane extracts ofS. aucuparia had the most potent anticancer activity in HeLa cells and should be considered as a potential clinical agent. The results support the ethnomedical claims for these species and suggest furtherin vitro and/orin vivo studies of the active extracts. Page(s): 43-49
Source: Indian Journal of Traditional Knowledge - Category: Science Source Type: research