Isolation and Characterization of a Novel Broad Host Range Bacteriophage Infecting Salmonella enterica subsp. enterica for Biocontrol and Rapid Detection.

In this study, a Salmonella Enteritidis-specific bacteriophage, KFS-SE1, was isolated, identified, and characterized. This Siphoviridae phage infects S. Enteritidis with high specificity. This phage is highly stable under various pH (5-11), temperature (4-60°C), and organic solvent conditions. The KFS-SE1 genome consists of 59,715-bp with 73 predicted open reading frames and 57.14% GC content; it has a complete set of genes required for phage reconstruction. Comparative phylogenetic analysis of KFS-SE1 revealed that it is very similar to the other Salmonella phages in the Siphoviridae family. These characteristics suggest that KFS-SE1 with high specificity and host lysis activity toward S. Enteritidis may have various potential applications. PMID: 29156510 [PubMed - as supplied by publisher]
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research