Determination of complex subclonal structures of hematological malignancies by multiplexed genotyping of blood progenitor colonies

Next-generation sequencing (NGS) methods have provided unprecedented insights into the somatic mutations associated with hematological malignancies, including myeloproliferative neoplasms (MPNs) [1 –3]. However, although we are now able to acquire detailed lists of mutations present in tumors at a given state of development, we are only beginning to understand how these mutations are associated in tumor subclones and the history of mutation acquisition during tumor development. The relevance of analyzing the makeup of tumors in detail has been demonstrated recently in studies of MPN patients and have shown, for the first time in any cancer, that the order in which somatic mutations are acquired influences tumor biology and clinical presentation [4,5].

Source: Experimental Hematology - October 9, 2017 Category: Hematology Authors: Francesca L. Nice, Charlie E. Massie, Thorsten Klampfl, Anthony R. Green Tags: Brief Communications Source Type: research