Pulsed cavitational therapy using high-frequency ultrasound for the treatment of deep vein thrombosis in an in vitro model of human blood clot.

Pulsed cavitational therapy using high-frequency ultrasound for the treatment of deep vein thrombosis in an in vitro model of human blood clot. Phys Med Biol. 2017 Oct 20;: Authors: Goudot G, Mirault T, Arnal B, Boisson-Vidal C, Le Bonniec B, Gaussem P, Galloula A, Tanter M, Messas E, Pernot M Abstract Post-thrombotic syndrome, a frequent complication of deep venous thrombosis can be reduced with early vein recanalization. Pulsed cavitational therapy (PCT) using ultrasound is a recent non-invasive approach. We propose to test the efficacy and safety of high-frequency focused PCT for a drug-free thrombolysis (thrombotripsy) in a realistic in vitro model of venous thrombosis.
 To reproduce venous thrombosis conditions, human whole blood was allowed to clot by stasis in silicone tubes (6 mm internal diameter) at a 30 cmH20 pressure, maintained during the whole experiment. We engineered an ultrasound device composed of dual 2.25 MHz transducers centered by a 6 MHz imaging probe. Therapeutic focus was generated at 3.2 cm depth from the probe. Thrombotripsy was performed by longitudinal scanning the thrombus at 3 different speeds: 1 mm.s-1 (n=6); 2 mm.s-1 (n= 6); 3 mm.s-1 (n= 12). Restored outflow was measured every 3 passages. Filters were placed to evaluate the debris size.
 24 occlusive thrombi, of 2.5 cm mean length and 4.4 kPa mean stiffness, were studied. Flow restoration was systematically obtained by 9 subsequent pa...
Source: Physics in Medicine and Biology - Category: Physics Authors: Tags: Phys Med Biol Source Type: research