Adherence of Ornithobacterium rhinotracheale to chicken embryo lung cells as a pathogenic mechanism.

Adherence of Ornithobacterium rhinotracheale to chicken embryo lung cells as a pathogenic mechanism. Avian Pathol. 2017 Oct 10;:1-22 Authors: De la Rosa-Ramos MA, Muñoz-Solís K, Palma-Zepeda M, Gutierrez-Castillo AC, López Villegas EO, Guerra-Infante FM, Castro-Escarpulli G Abstract Ornithobacterium rhinotracheale (O.rhinotracheale), is a bacterium that causes respiratory disease in birds and it has been isolated in countries with a large poultry production, including Mexico. The pathogenicity mechanisms of this bacterium have not been completely elucidated yet. The capacity of the bacterium to adhere to epithelial cells of chicken in vitro has been evidenced, and since this bacterium has been isolated from the lungs and air sacs of several avian species, the aim of this study was to determine if this bacterium can adhere to chicken lung cells. We used five O.rhinotracheale reference serovars (A-E) that were in contact with cells of a primary lung cells culture of a 19-day-old chicken embryo.O.rhinotracheale adherence was evaluated through optical and transmission electron (TEM) microscopies. The results revealed that O.rhinotracheale is capable of adhering to chicken embryo lung cells within 3 hours of incubation with a diffuse adherence pattern. The adherence percentages of the chicken embryo lung cells were 51% to 96% according to the serovar of the bacterium. Relative adherence was from 4 to 8 bacteria per cell. TEM data revea...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research