Mn3+-saturated bovine lactoferrin asa new complex with potential prebiotic activities for dysbiosis treatment and prevention – On the synthesis, chemical characterization and origin of biological activity

Publication date: November 2017 Source:Journal of Functional Foods, Volume 38, Part A Author(s): Klaudyna Śpiewak, Grzegorz Majka, Magdalena Pilarczyk- Żurek, Paweł M. Nowak, Michał Woźniakiewicz, Piotr Pietrzyk, Tomasz Korzeniak, Anna Stochel-Gaudyn, Krzysztof Fyderek, Magdalena Strus, Małgorzata Brindell A new bioactive compound, Mn3+-saturated bovine lactoferrin (Mn2-bLf) was obtained. It was stable under physiological conditions (pH≈7.5) although gradually releases Mn2+ ions under the simulated intestinal conditions (pH ≈ 6.5). This feature might be utilized for controlled delivery of Mn2+ ions to biological targets, among others to the probiotic strains of lactic acid bacteria whose growth is manganese-dependent. The Mn3+ modified bLf stimulates the growth of standard Lactobacillus plantarum and Lactobacillus rhamnosus, as well as the strains isolated from commercially available probiotic preparations, while the naturally occurring Fe-free and Fe-saturated forms of this protein are inactive. The uptake of manganese by Lactobacilli from Mn2-bLf added to culture supernatant was confirmed. Mn2-bLf was non-cytotoxic for Caco-2, CT-26 and HT-29 intestinal epithelial cells even up to 10mg/ml concentration. Mn2-bLf due to stimulation of the growth of Lactobacilli and lack of toxicity, can be considered asan innovative ingredient of the prebiotic composition.
Source: Journal of Functional Foods - Category: Nutrition Source Type: research